Degradation of chlorpyrifos in contaminated soil by immobilized laccase

Xin Wang, Meng Qin Yao, Li Liu, Yu Cao, Jia Bao

Abstract


In the present study, the embedding-crosslinking method was used to immobilize fungal laccase, and to determine the suitable conditions for immo­bilization by measuring various activities of the immobilized laccase. In addi­tion, the immobilized laccase was further employed to repair chlorpyrifos-con­taminated soil, and then the degradation rates of chlorpyrifos were measured under different conditions. Based on the results, the appropriate conditions for the method of embedding-crosslinking were found to be as follows: concen­tra­tion of sodium alginate – 3 %, concentration of glutaraldehyde – 1 %, cross­link­ing time – 6h, volume of crude laccase – 60 ml, and immobilization time – 4 h. After 48 h, the degradation rate of chlorpyrifos in soil could reach at least 70 % by the use of immobilized laccase that was prepared by the method of embedding-crosslinking under different environmental conditions.


Keywords


fungal; immobilization; embedding-crosslinking; degradation rate

References


M. J. Gorman, L. I. Sullivan, T. D. T. Nguyen, H. Dai, Y. Arakane, N. T. Dittmer, L. U. Syed, J. Li, D. H. Hua, M.R.Kanost, Insect Biochem. Mol. Biol. 42 (2012) 193

M. Lang, M. R. Kanost, M. J. Gorman, PLoS ONE 7 (2012) e33985

T. D. H. Bugg, M. Ahmad, E. M. Hardiman, R. Singh, Curr. Opin. Biotechnol. 22 (2011) 394

W. Bao, D. M. O’Malley, R. Whetten, R. R. Sederoff, Science 260 (1993) 672

A. Assavaing, B. Amornkitticharoen, N. Ekpasisal, V. Meevootisom, T. W. Flegel, Appl. Microbiol. Biotechnol. 38 (1992) 198

L. Jonsson, K. Sjostrom, I. Haggstrom, P. O. Nyman, Biochim. Biophys. Acta 1251 (1995) 210

L. Rehmann, E. Ivanova, Green Chem. 16 (2014) 1462

H. Noureddini, X. Gao, S. Joshi, P. R. Wagner, J. Oil Fat Ind. 79 (2002) 33

S. Sato, T. Murakata, M. Ochifuji, M. Fukushima, T. Suzuki, J. Chem. Eng. Jpn. 27 (1994) 732

K. Kawakami, S. Yoshida, Biotechnol. Tech. 9 (1995) 701

K. Kawakami, Biotechnol. Tech. 10 (1996) 491

K. Kawakami, S. Yoshida, J. Ferment. Bioeng. 82 (1996) 239

J .B. Zhang, Z. Q. Xu, H. Chen, Y. R. Zong, Biochem. Eng. J. 45 (2009)54

J. Zhang, Y. Yu, J. Ren, B. S. Wang, Adv. Mater. Res. 113–116 (2010) 2115

V. A. Abelyan, Appl. Biochem. Microbiol. 36 (2000) 310

P. S. R. Babu, T. Panda, Biotechnol. Tech. 5 (1991) 227

W. Y. Yang, M. Thirumavalavan, J. F. Lee, J. Membrane. Biol. 248 (2015) 231

J.Y. Kim, J. H. Yoon, D. H. Kim, S. B. Kim, Korean J. Chem. Eng. 29 (2012) 503

S. L. Manocha, Histochem. J. 2 (1970) 249

M. K. Morgan, L. S. Sheldon, C. W. Croghan, P. A. Jones, G. L. Robertson, J. C. Chuang, N. K. Wilson, C. W. Lyu, J. Expo. Anal. Environ. Epidemiol. 15 (2005) 297

A. L. Crane, R. G. Abdel, A. A. Ismail, O. Hendy, M. R. Bonner, M. R. Lasarev, M.

Al-Batanony, S. T. Singleton, K. Khan, J. R. Olson, D. S. Rohlman, J. Expo. Sci. Environ. Epidemiol. 23 (2013) 356

M. Saunders, B. L. Magnanti, S. C. SCorreia, A. Yang, U. Alamo-Hernandez, H. Riojas-Rodriguez, G. Calamandrei, J. G. Koppe, M. Krayer von Krauss, H. Keune, A. Bartonova, Environ. Health. 11 (2012) 1

R. O. Cristóvão, A. P. M. Tavarea, A. I. Brígida, J. M. Loureiro, A. R. B. Rui, A. M. Eugénia, A. Z. C. Maria, J. Mol. Catal., B: Enzym. 72 (2011) 6

L. Yang, Y. H. Zhao, B. X. Zhang, C. H. Yang, X. Zhang, FEMS Microbiol. Lett. 251 (2005) 67

R. R. M. Thengodkar, S. Sivakami, Biodegradation 21 (2010) 637

A. A. Romeh, M. Y. Hendawi, Environ. Chem. Lett. 11 (2013) 163

K. K. Dubey, M. H. Fulekar, J. Microbiol. Biotechnol. 28 (2012) 1715.




DOI: http://dx.doi.org/10.2298/JSC160128066W

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 J. Serb. Chem. Soc.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

IMPACT FACTOR 0.822 (131 of 166 journals)
5 Year Impact Factor 1.015 (118 of 166 journals)