HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil

Authors

  • Jovana Stanković Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade
  • Miroslav Novaković Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade
  • Vele Tešević University of Belgrade – Faculty of Chemistry, Studentski trg 12–16, 11000 Belgrade
  • Ana Ćirić Institute for Biological Research “Siniša Stanković”, University of Belgrade, Department of Plant Physiology, Bulevar despota Stefana 142,11000 Belgrade
  • Marina Soković Institute for Biological Research “Siniša Stanković”, University of Belgrade, Department of Plant Physiology, Bulevar despota Stefana 142,11000 Belgrade
  • Gordana Zdunić 4Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade
  • Zora Dajić-Stevanović Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Zemun
  • Dejan Gođevac Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade

DOI:

https://doi.org/10.2298/JSC190513106S

Keywords:

antimicrobial activity, direct bioautography, antibiofilm, antiquorum potential

Abstract

This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.

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Published

2020-01-10

How to Cite

[1]
J. Stanković, “HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil”, J. Serb. Chem. Soc., vol. 84, no. 12, pp. 1355-1365, Jan. 2020.

Issue

Section

Organic Chemistry

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