DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina Scientific paper

Main Article Content

Ivana Sofrenić
https://orcid.org/0000-0002-9799-670X
Boban Anđelković
https://orcid.org/0000-0002-9522-1492
Ljubodrag Vujisić
Miroslav Novaković
Aleksandar Knežević
https://orcid.org/0000-0002-2776-9675
Miroslava Stanković
Slobodan Milosavljević
https://orcid.org/0000-0002-0925-327X
Vele Tešević

Abstract

Eleven 31-methylenlanostane triterpenoids, i.e., seven 21- and four 26-oic acids, as well as a lupane triterpenoid betulin, isolated from the fruiting bodies of the mushroom Fomitopsis betulina, were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. Most of the tested com­pounds showed a beneficial effect by reducing DNA damage of human lym­pho­cytes more effectively than amifostine, a radioprotective agent, used as a positive control. All the tested compounds decreased MN frequency in the con­centration dependent manner, with the concentration of 2.0 µg mL-1 being the most effective – with increase of the concentration the activity slightly dec­reases. The structure–activity relationship (SAR) studies indicated that the lanostanes containing a conjugated 7,9 (11)-diene system exhibit lower activity than D8-analogues. It was also demonstrated that the DNA protective activities within the D8-lanostane-26-oic acid group are affected by the substitution in position 3 pattern. In the D8 series the oxygenation at C-12 or 16 as well as 21- or 26-oic acid functionality proved beneficial for in vitro protective effect on chromosomal aberrations. Betulin exhibited the lowest protective activity, but it is still comparable to that of amifostine.

Article Details

How to Cite
[1]
I. Sofrenić, “DNA protective activity of triterpenoids isolated from medicinal mushroom Fomitopsis betulina: Scientific paper”, J. Serb. Chem. Soc., vol. 86, no. 9, pp. 809-817, Aug. 2021.
Section
Biochemistry & Biotechnology

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