Effective valorisation of barley bran for simultaneous cellulase and β-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application

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Katarina R. Mihajlovski
Slađana Z. Davidović
Đorđe N. Veljović
Milica B. Carević
Vesna M. Lazić
Suzana I. Dimitrijević-Branković

Abstract

The agricultural raw industry generates large amounts of annually by--products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and β-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and β-amylase production using barley bran and the application of the natural isolate Paeni­bacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL-1, avicelase 0.433 U mL-1 and β-amyl­ase 1.594U mL-1). Scanning electron microscopy showed degradation of the lignocellulosic–starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and β-amylase, could hydro­lyze cotton fibres and barley bran, respectively. The main products after enz­ym­atic hydrolysis of cotton fibres and barley bran, glucose (0.117 g gmat-1) and maltose (0.347 g gmat-1), were quantified by high performance liquid chromato­graphy (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is econo­m­ically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.

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How to Cite
[1]
K. R. Mihajlovski, S. Z. Davidović, Đorđe N. Veljović, M. B. Carević, V. M. Lazić, and S. I. Dimitrijević-Branković, “Effective valorisation of barley bran for simultaneous cellulase and β-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application”, J. Serb. Chem. Soc., vol. 82, no. 11, pp. 1223–1236, Dec. 2017.
Section
Biochemistry & Biotechnology
Author Biography

Đorđe N. Veljović, University of Belgrade, Faculty of Technology and Metallurgy, Department of Inorganic Chemical Technology, Karnegijeva 4, Belgrade

University of Belgrade, Faculty of Technology and Metallurgy, Department of Inorganic Chemical Technology, Karnegijeva 4, Belgrade

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