Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)


  • Sandra Sretko Bulatović University of Belgrade - Faculty of Chemistry, Studentski trg 16, 11158 Belgrade
  • Nenad Marić University of Belgrade - Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade
  • Tatjana Šolević Knudsen University of Belgrade, Institute of Chemistry, Technology and Metallurgy
  • Jelena Avdalović University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000 Belgrade
  • Mila Vladislav Ilić University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, 11000 Belgrade
  • Branimir Jovančićević University of Belgrade - Faculty of Chemistry, Studentski trg 16, 11158 Belgrade
  • Miroslav M Vrvić BREM GROUP Ltd., Str. Oslobođenja 39b, 11090 Belgrade



enhanced in situ bioremediation, hydrocarbon-contaminated groundwater, biodegradation


Due to their extensive use, petroleum hydrocarbons are among the most common groundwater contaminants. Compared to the traditional methods of physical pumping of contamination from the aquifer and subsequent treat­ment (i.e., pump and treat), bioremediation is an economically cost-effective technology. The aim of this remediation approach is to transform biologically contaminants, most often by microbiological activity, into non-toxic com­pounds. More precisely, it is an active remediation process that involves bio­stimulation (increase of aquifer oxygenation, addition of nutrients) and/or bio­augmentation (injection of a concentrated and specialized population of micro­organisms). Using both biostimulation and bioaugmentation, enhanced in situ groundwater bioremediation was applied at a hydrocarbon-contaminated site in Belgrade. The bioremediation treatment, applied over twelve months, was highly efficient in reducing the concentrations of total petroleum hydrocarbon (TPH) to acceptable levels. The concentration of TPH in the piezometer P-5 was reduced by 98.55 %, in the piezometer P-6 by 98.30 % and in the piezo­meter P-7 by 98.09 %. These results provided strong evidence on the potential of this remediation approach to overcome site-limiting factors and enhance microbiological activity in order to reduce groundwater contamination.


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How to Cite

S. S. Bulatović, “Bioremediation of groundwater contaminated with petroleum hydrocarbons applied at a site in Belgrade (Serbia)”, J. Serb. Chem. Soc., vol. 85, no. 8, pp. 1067-1081, Aug. 2020.



Environmental Chemistry

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