Temperature dependent effect of difenoconazole on enzymatic activity from soil

MARIOARA NICOLETA FILIMON, SORIN OCTAVIAN VOIA, DIANA LARISA VLADOIU, ADRIANA ISVORAN, VASILE OSTAFE

Abstract


The purpose of this study was to quantify the effect of difenoconazole (DFC) on the activity of a few enzymes commonly found in soil: dehydrogenase, urease, phosphatase and protease. Three experimental variants were established: under field conditions with variable temperature (10–21 °C, variants A1–A3), under laboratory conditions with constant temperature (30 °C, variants B1–B3) and untreated soil (C variant). The commercial product “Score 250EC” with 250 g DFC L-1 was used at the following concentrations: 0.037 mg DFC g-1 soil (variants A1 and B1), 0.075 mg DFC g-1 soil (variants A2 and B2) and 0.150 mg DFC g-1 soil (variants A3 and B3). The dehydrogenase, phosphatase and urease activities decreased significantly (p < 0.05) under both field (variants A1–A3) and laboratory (variants B1–B3) conditions compared to untreated soil (variant C). The protease activity was reduced in variants A1–A3 compared to variant C and increased at the dose of 0.150 mg DFC g-1 soil in the variant B3.

Keywords


fungicide; soil; dehydrogenase; phosphatase; urease; protease

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DOI: https://doi.org/10.2298/JSC141218030F

Copyright (c) 2015 Journal of the Serbian Chemical Society - J. Serb. Chem. Soc.

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