@article{Shroff_Mohanta_Baitharu_Bag_Behera_2022, place={Belgrade, Serbia}, title={Microwave assisted synthesis of novel spiro diarylidenes and their antimicrobial assay: Scientific paper}, volume={87}, url={https://shd-pub.org.rs/index.php/JSCS/article/view/10319}, DOI={10.2298/JSC210123031S}, abstractNote={<p>A rapid and high yield microwave assisted synthesis of a series of novel 7,9-bis-(arylidene)-4-methyl-2,6,10-triphenyl-2,3-diazaspiro[4,5]dec-3-ene-1,8-dione has been explored. The spiro diarylidene derivatives having nit­rogen atom and <em>α</em>,<em>β</em>-unsaturated ketone moiety were synthesized by aldol con­densation between 4-methyl-2,6,10-triphenyl-2,3-diazaspiro[4,5]dec-3-ene-1,8-dione and corres­pon­d­ing aryl aldehydes followed by dehydration. The syn­thesized series of novel spirodiarylidene derivatives were characterized using IR, <sup>1</sup>H- and <sup>13</sup>C-NMR and mass spectra. Density functional theory (DFT) study was performed by Gaussian 09 software. The antimicrobial activities of the synthesized derivatives were evalu­ated against two pathogenic Gram-positive and Gram-negative bacterial strain and three pathogenic fungal species by disk diffusion method<em>. </em>The minimum inhi­bitory concentration was determined by the microbroth dilution technique. The results of the present study demon­strated that the examined compounds marked <strong>5a </strong>and<strong> 5c</strong>, possessing 4-NO<sub>2</sub> and 5-Br-2-OH substituents, are found to be more active against Gram-positive bacterium <em>Staphylococcus aureus</em>, 8 and 16 µg mL<sup>-1</sup>, respectively, and moder­ately active against Gram-negative bacteria<em> Pseudomonas aeruginos</em><em>a</em> and <em>Escherichia col</em><em>i</em>, compared to other synthetic derivatives. However, none of the synthesized derivatives showed any activity against <em>Streptococcus pyro­genes</em>. Compound <strong>5e</strong>, possessing 2,4,6-(OCH<sub>3</sub>)<sub>3</sub>C<sub>6</sub>H<sub>3</sub> moiety, exhibited broad spectrum activity against all fungal strains under study, but showed no anti­bacterial activity.</p>}, number={7-8}, journal={Journal of the Serbian Chemical Society}, author={Shroff, Sabita and Mohanta, Prajna Parimita and Baitharu, Iswar and Bag, Bhawani Prasad and Behera, Ajay Kumar}, year={2022}, month={May}, pages={813–827} }