@article{Mitrev_Mehandzhiyski_Batovska_Liese_Galunsky_2016, place={Belgrade, Serbia}, title={Original enzyme-catalyzed synthesis of chalcones: Utilization of hydrolase promiscuity}, volume={81}, url={https://shd-pub.org.rs/index.php/JSCS/article/view/2863}, DOI={10.2298/JSC160422069M}, abstractNote={<p class="Abstrakt"><span lang="EN-US">An </span><em><span lang="EN-US">E</span></em><span lang="EN-US">-chalcone was obtained with very high stereoselectivity for the first time by an enzyme-catalyzed Claisen–Schmidt condensation between benzaldehyde and acetophenone. From a set of lipases, only that from hog pancreas demonstrated promiscuity, catalyzing the reaction in the presence of imidazole as a promoter. Another enzyme, acylase from <em>Aspergillus melleu</em>s (EC 3.5.1.14) also proved to be active in the synthesis of <em>E-</em>chalcone under the same reaction conditions. This acylase along with the recombinant </span><span lang="EN-US">D</span><span lang="EN-US">-amino­acylase (EC 3.5.1.81) also catalyzed the reaction between acetophenone and <em>p</em>-nitrobenzaldehyde. Such a "green" approach to the synthesis of chalcones is of great interest because of the important applications of chalcones as formula ingredients in the pharmaceutical, food and cosmetic industries.</span></p>}, number={11}, journal={Journal of the Serbian Chemical Society}, author={Mitrev, Yavor N and Mehandzhiyski, Aleksander Y and Batovska, Daniela I and Liese, Andreas and Galunsky, Boris}, year={2016}, month={Nov.}, pages={1231–1237} }