Statistical optimization of lipase production from oil mill effluent by Acinetobacter sp. KSPE71 Scientific paper

Abstract

The present study investigated the valorisation of oil-rich residues of coconut oil mill effluent (COME) as a potential growth medium for the micro­bial production of extracellular lipase. The bacterial species isolated from oil mill effluent, Acinetobacter sp. KSPE71 was tested for its efficiency to grow and produce lipase in undiluted COME and 0.2 % yeast extract and 0.2 % NH4Cl sup­plemented COME. In this connection, the process parameters such as pH, tem­pe­rature, agitation speed, and inoculum size were optimized to max­i­mize the produc­tion using a central composite design in the Response surface methodology. At the optimized state of pH 7.5, 35 °C, 150 rpm with 0.6 % ino­culum size, a maximum of 3.95 U mL^-1 activity was obtained, four-fold higher than the basal condition. At this stage, 73 % of the lipid content was degraded. The present work results imply that the oil mill effluent can be used as a cheaper production medium for lipase and the new isolate Acinetobacter sp. KSPE71 as a potential lipase pro­ducer. The degradation of oil waste along with the production of the valuable pro­duct has multiple advantages of cost reduct­ion of lipase and environmental concern.