Analyzing food supplements to enhance erectile system function for the presence of undeclared sildenafil and tadalafil using HPLC-DAD
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Abstract
Phosphodiesterase-5 (PDE-5) inhibitors, such as sildenafil and tadalafil, are commonly used drugs to improve erectile function. However, some erectile function-enhancing supplements with claims of being entirely natural may be adulterated with these drugs, posing health risks. This study screened supplements for erectile dysfunction from the Republic of North Macedonia using a fast, specific, and sensitive HPLC-DAD method (runtime: 15 min). No interference was observed at the retention times of sildenafil and tadalafil, allowing for their unambiguous identification and quantification. Tadalafil was absent, but sildenafil was found in two of eight samples (32.78 mg and 13.47 mg per dosage). The results demonstrated the applicability of the method to determine whether food supplements marketed for erectile function enhancement are free of sildenafil and tadalafil.
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