Characterization of some naphthalene utilizing bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)

Sancho Rajan, Anwesha Pattanaik, Venkatesh Kumaresan, Prasanth Bhatt, Senthilarasu Gunasekaran, Jesu Arockiaraj, Mukesh Pasupuleti, Vladimir P Beskoski, Paromita Chakraborty

Abstract


Microorganisms capable of utilizing naphthalene as the sole carbon source were isolated from the contaminated sediment of Cooum River. Twenty one isolates were recovered and nine were selected for enrichment due to dif­fe­rences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D, NS19-SRMN­D14E) were capable of completely utilizing naphthalene as the sole source of carbon in Carbon Free Minimal Medium (CFMM) supplemented with naphtha­lene. 16s rDNA sequencing showed that all the four isolates were distantly related from each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRM­ND14D), and Sphingobacterium sp. (NS19-SRMND14E) respectively. Based on the phylogenetic analysis of 16s rDNA sequencing, the isolate Sphingo­bacterium sp. (NS19-SRMND14E) has been identified as novel strain. Poly­merase Chain Reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along the Bay of Bengal.

Keywords


naphthalene, indigenous bacteria, ndo gene, 16s RNA, remediation, phylogenetic tree

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DOI: https://doi.org/10.2298/JSC180724088R

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