Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)

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Published: Mar 8, 2019
DOI: https://doi.org/10.2298/JSC180724088R
Keywords:
naphthalene indigenous bacteria ndo gene 16S RNA remediation phylogenetic tree

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Sancho Rajan
Department of Civil Engineering, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Anwesha Pattanaik
SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Venkatesh Kumaresan
Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Prasanth Bhatt
Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Senthilarasu Gunasekaran
SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Jesu Arockiaraj
SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu
Mukesh Pasupuleti
Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow 226031
Vladimir P Beškoski
University of Belgrade-Faculty of Chemistry University of Belgrade, Studentski trg 12-16, 11000 Belgrade
Paromita Chakraborty
1RM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu and Department of Civil Engineering, SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu

Abstract

Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of CooumRiver. Twenty one isolates were recovered and nine were selected for enrichment due to dif­fe­rences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19-
-SRMN­D14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphtha­lene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRM­ND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingo­bacterium sp. (NS19-SRMND14E) has been identified as novel strain. Poly­merase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along theBay of Bengal.

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How to Cite
[1]
S. Rajan, “Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)”, J. Serb. Chem. Soc., vol. 84, no. 2, pp. 225–236, Mar. 2019.
Issue
Vol. 84 No. 2 (2019)
Section
Environmental Chemistry

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References

P. Goswami, T. Ohura, K. S. Guruge, M. Yoshioka, N. Yamanaka, M. Akiba, N. Munus¬wamy, Ecotox. Environ. Safe. 130 (2016) 113 (https://doi.org/10.1016/j.ecoenv.2016.04.016)

S. Veerasingam, R. Venkatachalapathy, S. Sudhakar, P. Raja, V. Rajeswari, J. Environ. Sci. 23 (2011) 1129 (https://doi.org/10.1016/S1001-0742(10)60524-4)

P. Nammalwar, P. Pakshirajan, in Proceedings of the IV National Symposium on "Environment", 1995, Madras, Anna University, India, p. 52

B. Prajapati, G. Shiv, S. Gaherwal, K. Billore, R. Soni, Int. J. Res. Chem. Environ. 6 (2016) 26

S. K. Samanta, O. V. Singh, R. K. Jain, Trends Biotechnol. 20 (2002) 243 (https://doi.org/10.1016/S0167-7799(02)01943-1)

R. Nagabhushanam, P. Machale, R. Katyayani, P. Reddy, R. Sarojini, J. Ecotoxicol. Environ. Monit. 1 (1991) 185

E. C. Santos, R. J. Jacques, F. M. Bento, R. P. Maria do Carmo, P. A. Selbach, E. L. Sá, F. A. Camargo, Bioresource Technol. 99 (2008) 2644 (https://doi.org/10.1016/j.biortech.2007.04.050)

D. Ghosal, S. Ghosh, T. K. Dutta, Y. Ahn, Front. Microbiol. 7 (2016) 1369 (https://doi.org/10.3389/fmicb.2016.01369)

B. M. Dellagnezze, G. V. de Sousa, L. L. Martins, D. F. Domingos, E. E. Limache, S. P. de Vasconcellos, G. F. da Cruz, V. M. de Oliveira, Mar. Pollut. Bull. 89 (2014) 191 (https://doi.org/10.1016/j.marpolbul.2014.10.003)

M. Hassanshahian, G. Emtiazi, G. Caruso, S. Cappello, Mar. Environ. Res. 95 (2014) 28 (https://doi.org/10.1016/j.marenvres.2013.12.010)

A. Pawar, S. Ugale, M. More, N. Kokani, S. Khandelwal, J. Bioremed. Biodeg. 4 (2013) 203 (https://doi.org/10.4172/2155-6199.1000203)

G. Gojgic-Cvijovic, J. Milic, T. Solevic, V. Beskoski, M. Ilic, L. Djokic, T. Narancic, M. Vrvic, Biodegradation 23 (2012) 1 (https://doi.org/10.1007/s10532-011-9481-1)

V. P. Beškoski, G. Gojgić-Cvijović, J. Milić, M. Ilić, S. Miletić, T. Šolević, M. M. Vrvić, Chemosphere 83 (2011) 34 (https://doi.org/10.1016/j.chemosphere.2011.01.020)

N. Ceyhan, Sci. Res. Essays 7 (2012) 66 (https://doi.org/10.5897/SRE11.1146)

C. Alquati, M. Papacchini, C. Riccardi, S. Spicaglia, G. Bestetti, Ann. Microbiol. 55 (2005) 237

T.-T. Liu, Y. Xu, H. Liu, S. Luo, Y.-J. Yin, S.-J. Liu, N.-Y. Zhou, Appl. Microbiol. Biot. 90 (2011) 671 (https://doi.org/10.1007/s00253-010-3033-1)

T. Basta, A. Keck, J. Klein, A. Stolz, J. Bacteriol. 186 (2004) 3862 (https://doi.org/10.1128/JB.186.12.3862-3872.2004)

J. K. Fredrickson, D. L. Balkwill, M. F. Romine, T. Shi, J. Ind. Microbiol. Biot. 23 (1999) 273 (https://doi.org/10.1038/sj/jim/2900741)

W.-Q. Zhuang, J.-H. Tay, A. Maszenan, S. Tay, Appl. Microbiol. Biot. 58 (2002) 547 (https://doi.org/10.1007/s00253-001-0909-0)

N. Kosaric, H. Choi, Tenside Surfact. Det. 27 (1990) 294

B. M. Nkem, N. Halimoon, F. M. Yusoff, W. L. W. Johari, M. P. Zakaria, S. R. Medipally, N. Kannan, Mar. Pollut. Bull. 107 (2016) 261 (https://doi.org/10.1016/j.marpolbul.2016.03.060)

G. S. Sayler, S. W. Hooper, A. C. Layton, J. H. King, Microb. Ecol. 19 (1990) 1 (https://doi.org/10.1007/BF02015050)

C. M. Serdar, D. T. Gibson, Biochem. Bioph. Res. Co. 164 (1989) 772

M. J. Simon, T. D. Osslund, R. Saunders, B. D. Ensley, S. Suggs, A. Harcourt, S. Wen-chen, D. L. Cruder, D. T. Gibson, G. J. Zylstra, Gene 127 (1993) 31

M. F. Romine, L. C. Stillwell, K.-K. Wong, S. J. Thurston, E. C. Sisk, C. Sensen, T. Gaasterland, J. K. Fredrickson, J. D. Saffer, J. Bacteriol. 181 (1999) 1585

E. Kim, G. Zylstra, J. Ind. Microbiol. Biot. 23 (1999) 294

L. A. Kulakov, S. Chen, C. C. Allen, M. J. Larkin, Appl. Environ. Microb. 71 (2005) 1754 (https://doi.org/10.1128/AEM.71.4.1754-1764.2005)

P. Khara, M. Roy, J. Chakraborty, D. Ghosal, T. K. Dutta, FEBS Open Bio. 4 (2014) 290 (https://doi.org/10.1016/j.fob.2014.03.001)

L. Zhou, H. Li, Y. Zhang, S. Han, H. Xu, Braz. J. Microbiol. 47 (2016) 271 (https://doi.org/10.1016/j.bjm.2016.01.001)

C. Hamann, J. Hegemann, A. Hildebrandt, FEMS Microbiol. Lett. 173 (1999) 255

D. Das, R. Baruah, A. S. Roy, A. K. Singh, H. P. D. Boruah J. Kalita, T. C. Bora, Genomics 105 (2015) 182 (https://doi.org/10.1016/j.ygeno.2014.12.006).