Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns
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Abstract
The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL-1 units, respectively), moderate adhesion to hexadecane (39±2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.
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References
FAO/WHO, Probiotics in Food. Health and Nutritional Properties and Guidelines for Evaluation, Food and Nutrition Paper, 2002, Vol. 85, pp. 1–56
S. Fijan, Antimicrobial effect of probiotics against common pathogens, in Probiotics and Prebiotics in Human Nutrition and Health, V. Rao, L. G. Rao, Eds., In Tech, Venka¬teswera, 2016 (on line, https://doi.org/10.5772/63141)
J. K. Kaushik, A. Kumar, R. K. Duary, A. K. Mohanty, S. Grover, V. K. Batish, PLoS One 4 (2009)e8099 (https://doi.org/10.1371/journal.pone.0008099)
C. De Champs, N. Maroncle, D. Damien, C. Rich, C. Forestier, J. Clin. Microbiol. 41 (2003) 1270 (doi: 10.1128/JCM.41.3.1270-1273.2003)
M. Fakruddin, M. N. Hossain, M. M. Ahmed, BMC Complementary Altern. Med. 17 (2017) 64 (https://doi.org/10.1186/s12906-017-1591-9)
P. Shokryazdan, C. C. Sieo, R. Kalavathy, J. B. Liang, N. B. Alitheen, M. F. Jahromi, Y. W. Ho, BioMed Res. Int. 2014 (2014), Article ID 927268 (http://dx.doi.org/10.1155/2014/927268)
R. J. Boyle, R. M. Robins-Browne, M. L. K. Tang, Am. J. Clin. Nutr. 83 (2006) 1256 (https://doi.org/10.1093/ajcn/83.6.1256)
M. E. Sanders, D. Merenstein, C. A. Merrifield, R. Hutkins. Nutr. Bull. 43 (2018) 212 (https://doi.org/10.1111/nbu.12334)
T. Dhewa, V. Bajpai, R. K. Saxena, S. Pant, V. Mishra, Int. J. Probiotics Prebiotic. 5 (2010) 45
M. van den Nieuwboer, E. Claassen, L. Morelli, F. Guarner, R. J. Brummer, Benefic. Microbes 5 (2014) 45 (https://doi.org/10.3920/BM2013.0046)
D. K. Dahiya, A. K. Puniya., J. Food Sci. Technol. 54 (2017) 792 (doi: 10.1007/s13197-017-2523-x)
A. K. Al Atya, D. K. Hadiouche, R. Ravallec, A. Silvain, A. Vachee, D. Drider, Front. Microbiol. 6 (2015) 227 (https://doi.org/10.3389/fmicb.2015.00227)
P. Kavitha, D. Sindhuja, M. Banumathi, Int. J. Curr. Microbiol. Appl. Sci. 5 (2016) 1042 (doi: http://dx.doi.org/10.20546/ijcmas.2016.504.119)
A. Lombardi, M. Gatti, L. Rizzoti, S. Torriani, C. Andrighetto, G. Giraffa, Int. Dairy J. 14 (2004) 967 (https://doi.org/10.1016/j.idairyj.2004.04.005)
V. S. Ocana, E. Bru, A. A. de Ruiz Holgado, M. E. Nader-Macias, J. Gen. Appl. Microbiol. 45 (1999) 203 (https://doi.org/10.2323/jgam.45.203)
L. J. Harris, M. A. Daeschel, M. E. Stiles, T. R. Klaenhammer, J. Food Prot. 52 (1989) 384 (https://doi.org/10.4315/0362-028X-52.6.384)
Y. S. Huh, Y. S. Jun, Y. K. Hong, H. Song, S. Y. Lee, W. H. Hong, Process Biochem. (Oxford, U.K.) 41 (2006) 1461
The Law on Experimental Animal Treatment, Official Gazette of the Republic of Serbia, No. 41/2009
Directive 2010/63/EU; European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (http://data.europa.eu/eli/dir/2010/63/oj)
European Pharmacopoeia 6.0, 2008, Abnormal toxicity, p. 165
J. S. Zhou, Q. Shu, K. J. Rutherfurd, J. Prasad, P. K. Gopal, H. S. Gill, Food Chem. Toxicol. 38 (2000) 153 (doi: 10.1016/S0278-6915(99)00154-4)
S. Oh, S. H. Kim, R. W. Worobo, J. Dairy Sci. 83 (2000) 2747 (https://doi.org/10.3168/jds.S0022-0302(00)75169-1)
D. I. Pereira, G. R. Gibson, Appl. Environ. Microbiol. 68 (2002) 4689 (doi:10.1128/AEM.68.9.4689-4693.2002)
M. Fernandez, S. Boris. C. Barbes, J. Appl. Microbiol. 94 (2003) 449 (https://doi.org/10.1046/j.1365-2672.2003.01850.x)
S. Fijan, Int. J. Environ. Res. Public Health 11 (2014) 4745 (doi: 10.3390/ijerph110504745)
M. Mikelsaar, M. Zilmer, Microb. Ecol. Health Dis. 21 (2009) 1 (https://doi.org/10.1080/08910600902815561)
M. S. Juarez-Tomas, V. S. Ocana, B. Wiese, M. E. Nader-Macias, J. Med. Microbiol. 52 (2003) 1117 (https://doi.org/10.1099/jmm.0.05155-0)
J. Flach, M. B. van der Waal, A. F. M. Kardinaal, J. Schloesser, R. M. A. J. Ruijschop, E. Claassen, Cogent Food Agric. 4 (2018) 1452839 (https://doi.org/10.1080/23311932.2018.1452839).