Interpretative optimization of the isocratic ion chromatographic separation of anions

Žaklina N. Todorović, Ljubinka V. Rajaković, Antonije E. Onjia

Abstract


Interpretive retention modeling was utilized to optimize the isocratic ion chromatographic (IC) separation of the nine anions (formate, fluoride, chloride, nitrite, bromide, nitrate, phosphate, sulfate and oxalate). The carbonate-bicarbonate eluent was used and separation was done on a Dionex AS14 ion-exchange column. The influence of combined effects of two mobile phase factors, the total eluent concentration (2–6 mM) and the carbonate/bicar­onate ratio from 1:9 to 9:1 (which correspond to pH range 9.35–11.27), on the IC separation was studied. The multiple species analyte/eluent model that takes into account ion-exchange equilibria of the eluent and sample anions was used. In order to estimate the parameters in the model, a non-linear fitting of the retention data, obtained at two-factor three-level experimental design, was applied. To find the optimal conditions in the experimental design, the nor­mal­ized resolution product as a chromatographic objective function was employed. This criterion includes both the individual peak resolution and the total analysis time. A good agreement between experimental and simulated chromatograms was obtained.


Keywords


anions; ion chromatography; isocratic; multiple species analyte/ /eluent; resolution product

Full Text:

PDF (2,412 kB)


DOI: https://doi.org/10.2298/JSC150927022T

Copyright (c) 2016 Journal of the Serbian Chemical Society - J. Serb. Chem. Soc.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

IMPACT FACTOR 0.828 (140 of 172 journals)
5 Year Impact Factor 0.917 (140 of 172 journals)