An effective and facile approach for the determination of bioactive components of Rheum ribes in the Kurdish state of Iraq and Siirt region in Turkey
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Abstract
Developing a streamlined and accessible method for identifying the bioactive components of Rheum ribes (rhubarb) holds significant promise in unlocking its therapeutic potential and advancing research in natural medicine. In this study, bioactive components of rhubarb such as total phenolics and flavonoids as well as the antioxidant activity of its methanolic extract were determined. Total phenolic content was between 84.02 and 387.53 mg/L gallic acid equivalent (GAE) in extracts. Total flavonoid contents determined by the aluminium chloride colorimetric method ranged from 69.98 to 935.75 mg L-1 of routine equivalents (RE) in the extracts. The antioxidant activities were determined using ferric reducing antioxidant potential (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. In the FRAP assay, the highest antioxidant activity (IC50) was found as 25.18 ± 0.04 mg L-1 extract. In the DPPH method, the maximum percentage inhibition was found as 88.11 %. Iron chelating activities of the samples were above 70 %. The chemical compound contents of the extracts were determined by LC-MS/MS. In this step, a total of 25 phenolic and flavonoid compounds in extracts were analyzed qualitatively and quantitatively. Malic acid (15.72 ± 0.53 mg kg-1) and rutin (76.93 ± 0.03 mg kg-1) in the extract were identified as the major phytochemicals compounds. The results of the study confirm that rhubarb have potential biological activities and can be introduced as an important sources of natural antioxidants.
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References
E. Nieboer, D. H. S. Richardson, Environ. Pollution. Ser. B, Chem. Phys. 1 (1980) 3–26 (https://doi.org/10.1016/0143-148X(80)90017-8)
D. M. Sabir, J. Zankoy Sulaimani - Part A 3 (2000) 15–21 (https://doi.org/10.17656/jzs.10049)
J. U. Lloyd, Origin and History of All the Pharmacopeial Vegetable Drugs, Chemicals and Preparations with Bibliography, Read Books, 2008.
Q. Zheng, H. Wu, J. Guo, H. Nan, S. Chen, J. Yang, X. XuU, Chinese Herb. Med. 5 (2013) 9–32 (https://doi.org/10.7501/j.issn.1674-6384.2013.01.003)
R. K. Sindhu, P. Kumar, J. Kumar, A. Kumar, S. Arora, Int. J. Pharm. Pharm. Sci. 2 (2010) 90–92
W. Sun, M. H. Shahrajabian, Molecules 28 (2023) 1845 (https://doi.org/10.3390/molecules28041845)
H. Tsuchiya, M. Sato, T. Miyazaki, S. Fujiwara, S. Tanigaki, M. Ohyama, T. Tanaka, M. Iinuma, J. Ethnopharmacol. 50 (1996) 27–34 (https://doi.org/10.1016/0378-8741(96)85514-0)
Ali al-Rawi and H. L. Chakravarty, Medical plant of Iraq, Edn 2, Government Press, Baghdad, 1964.
N. M. Aziz, Chem. Mater. Res. 3 (2013) 27–32 (https://www.iiste.org/Journals/index.php/CMR/article/view/9384/9606)
P. Xiao, L. He, L. Wang, J. Ethnopharmacol. 10 (1984) 275–293 (https://doi.org/10.1016/0378-8741(84)90016-3)
N. Bagheriani, M. Bahrami, M. Kamalinejad, Z. Rampisheh, M. Kashanian, E. Akhtari, Res. J. Pharmacogn. 10 (2023) 41–50 (https://doi.org/10.22127/RJP.2022.342450.1896)
T. Hartmann, Phytochemistry 68 (2007) 2831–2846. doi:10.1016/j.phytochem.2007.09.017.
A. U. Arvindekar, K. S. Laddha, Ind. Crops Prod. 83 (2016) 587–595 (https://doi.org/10.1016/j.indcrop.2015.12.066)
Y. Shikishima, Y. Takaishi, G. Honda, M. Ito, Y. Takeda, O. K. Kodzhimatov, O. Ashurmetov, Phytochemistry 56 (2001) 377–381 (https://doi.org/10.1016/S0031-9422(00)00370-8)
V. Rajkumar, G. Guha, R. Ashok Kumar, Evidence-Based Complement. Altern. Med. 2011 (2011) 1–9 (https://doi.org/10.1093/ecam/neq048)
L. Krenn, A. Presser, R. Pradhan, B. Bahr, D. H. Paper, K. K. Mayer, B. Kopp, J. Nat. Prod. 66 (2003) 1107–1109 (https://doi.org/10.1021/np0301442)
P. L. Kuo, T. C. Lin, C. C. Lin, Life Sci. 71 (2002) 1879–1892 (https://doi.org/10.1016/S0024-3205(02)01900-8)
M. A. Yilmaz, A. Ertas, I. Yener, M. Akdeniz, O. Cakir, M. Altun, I. Demirtas, M. Boga, H. Temel, J. Pharm. Biomed. Anal. 154 (2018) 413–424 (https://doi.org/10.1016/j.jpba.2018.02.059)
M. A. Yilmaz, Ind. Crops Prod. 149 (2020) 112347 (https://doi.org/10.1016/j.jpba.2018.02.059)
M. Fidan, İ. Teğin, M. E. Erez, S. M. Pınar, H. Eroğlu, Acad. Platf. J. Eng. Sci. 8 (2020) 41–48 (https://doi.org/10.21541/apjes.510659)
I. Tegin, G. Canpolat, M. Fidan, The Antioxidant Capacity, Total Phenolic Content and Phenolic Compounds of Spergularia rubra (L.) J. Presl & C. Presl in Naturally Distributed Five Different Saline Areas in Siirt Province, in 2018 2nd Int. Symp. Multidiscip. Stud. Innov. Technol., IEEE, 2018, pp. 1–4 (https://doi.org/10.1109/ISMSIT.2018.8567312)
I. Tegin, G. Canpolat, M. Fidan, The Antioxidant Capacity, Total Phenolic Content and Phenolic Compounds of Plantago coronopus L. subsp. coronopus in Naturally Distributed in Akdoǧmuş-Siirt, in ISMSIT 2018 - 2nd Int. Symp. Multidiscip. Stud. Innov. Technol. Proc., Institute of Electrical and Electronics Engineers Inc., 2018. (https://doi.org/10.1109/ISMSIT.2018.8567312)
İ. Tegin, E. Yabalak, B. Sadik, M. Fidan, Rev. Roum. Chim. 64 (2019) 673–679 (http://revroum.lew.ro/wp-content/uploads/2019/08/Art%2004.pdf)
M. Boğa, A. Ertaş, M. A. Yılmaz, M. Kızıl, B. Çeken, N. Haşimi, T. Y. Özden, S. Demirci, İ. Yener, Ö. Deveci, Iran. J. Pharm. Res. 15 (2016) 393–405 (https://pmc.ncbi.nlm.nih.gov/articles/PMC5149026/)
M. A. Yılmaz, Determining The Metabolic Profile Of Some Achıllea Species By LC-MS- IT-TOF AND LC-MS/MS And Investigatıon Of Their Biological Activities, Dicle Unıversıty Instıtute of Natural and Applıed Scıences, Diyarbakır, Türkiye, 2015.
S L R Ellison (LGC, UK), A Williams (UK), eds., Eurachem/CITAC guide: Quantifying Uncertainty in Analytical Measurement, Third Edit, 2019 (https://www.eurachem.org/images/stories/Guides/pdf/QUAM2012_P1.pdf)
L. Yu, S. Haley, J. Perret, M. Harris, Food Chem. 78 (2002) 457–461 (https://doi.org/10.1016/S0308-8146(02)00156-5)
İ. Tegin, E. Yabalak, B. Hallaç, N. Sabancı, M. Fidan, B. Sadik, Int. J. Environ. Health Res. (2024) 1–18 (https://doi.org/10.1080/09603123.2024.2382304)
E. A. Ibrahim, D. H. A. Baker, F. K. El-Baz, Int. J. Pharm. Sci. Rev. Res. 39 (2016) 93–99 (http://globalresearchonline.net/journalcontents/v39-2/17.pdf)
J. Zhishen, T. Mengcheng, W. Jianming, Food Chem. 64 (1999) 555–559 (https://doi.org/10.1016/S0308-8146(98)00102-2)
Y. Zou, Y. Lu, D. Wei, J. Agric. Food Chem. 52 (2004) 5032–5039 (https://doi.org/10.1021/jf049571r)
D. Villaño, M. S. M. S. Fernández-Pachón, M. L. M. L. Moyá, A. M. A. M. Troncoso, M. C. C. M. C. García-Parrilla, Talanta 71 (2007) 230–235 (https://doi.org/10.1016/j.talanta.2006.03.050)
A. Ertas, M. Boga, M. A. Yilmaz, Y. Yesil, G. Tel, H. Temel, N. Hasimi, I. Gazioglu, M. Ozturk, P. Ugurlu, Ind. Crops Prod. 67 (2015) 336–345 (https://doi.org/10.1016/j.indcrop.2015.01.064)
I. F. F. Benzie, J. J. Strain, Anal. Biochem. 239 (1996) 70–76 (https://doi.org/10.1006/abio.1996.0292)
M. Öztürk, F. Aydoǧmuş-Öztürk, M. E. Duru, G. Topçu, Food Chem. 103 (2007) 623–630 (https://doi.org/10.1016/j.foodchem.2006.09.005)
F. Menaa, A. Menaa, J. Tréton, Polyphenols against Skin Aging, in Polyphenols in Human Health and Disease (R. R. Watson, V. R. Preedy, S. Zibadi, Eds), Elsevier, 2014, pp. 819–830 (https://doi.org/10.1016/B978-0-12-398456-2.00063-3)
İ. Yener, Ö. T. Ölmez, A. Ertas, M. A. Yilmaz, M. Firat, S. İ. Kandemir, M. Öztürk, U. Kolak, H. Temel, Ind. Crops Prod. 123 (2018) 442–453 (https://doi.org/10.1016/j.indcrop.2018.07.007)
S. Habtemariam, Nat. Prod. Commun. 6 (2011) 1934578X1100600 (https://doi.org/10.1177/1934578X1100600211)
Y. Wang, C. Tang, H. Zhang, J. Food Drug Anal. 23 (2015) 310–317 (https://doi.org/10.1016/j.jfda.2014.10.002)
A. A. Zanwar, S. L. Badole, P. S. Shende, M. V. Hegde, S. L. Bodhankar, Cardiovascular Effects of Hesperidin, in Polyphenols Hum. Heal. Dis., Elsevier, 2014, pp. 989–992. doi:10.1016/B978-0-12-398456-2.00076-1.
S. H. Lee, Y. B. Park, K. H. Bae, S. H. Bok, Y. K. Kwon, E. S. Lee, M. S. Choi, Ann. Nutr. Metab. 43 (1999) 173–180 (https://doi.org/10.1159/000012783)
Y. Nahmias, J. Goldwasser, M. Casali, D. van Poll, T. Wakita, R. T. Chung, M. L. Yarmush, Hepatology 47 (2008) 1437–1445 (https://doi.org/10.1002/hep.22197).