An investigation of the influence of pH and ionic strength on the adsorption and interfacial dilatational properties at the oil–water interface of pumpkin (Cucurbita pepo) seed protein hydrolysate

Sandra Đ. Bučko, Jaroslav M. Katona, Lidija B. Petrović, Jelena R. Milinković, Jadranka L. Fraj, Ljiljana M. Spasojević, Reinhard Miller

Abstract


Pumpkin (Cucurbita pepo) seed protein hydrolysate (PSPH) was obtained by enzymatic hydrolysis of pumpkin seed protein isolate using pepsin. Influence of pH (3, 5 and 8) and ionic strength, Ic (0–1 mol dm-3), on the adsorption kinetics of PSPH (diffusion rate constant, kdiff, and adsorption rate constant, kads), interfacial pressure (π) and interfacial dilatational properties (dilatational elasticity, E′, and viscosity, E″) of the oil–PSPH solution inter­faces was investigated at different PSPH concentrations (c = 0.0014–14 g dm-3). It was found that PSPH adsorbs to the interface at c ˃ 0.0014 g dm-3, regardless of pH and ionic strength, as evidenced by the increase in interfacial pressure. The kdiff and kads value were found to be the highest at pH 3 and the lowest at pH 5 at the corresponding concentrations. The dilatational properties of the inter­faces, which were investigated at different oscillation frequencies, ν, 0.01–0.2 Hz, showed that the E′ of the oil–PSPH solution interfaces is much higher than its E″. Moreover, E′ increases with increasing PSPH concentration at pH 5 and 8, and with increasing Ic, regardless of the pH, while E″ changes only mini­mally.


Keywords


natural surfactants; enzymatic hydrolysis; oil–water interface; interfacial dilatational rheology; dilatational elasticity

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References


F. Ravera, G. Loglio, V. I. Kovalchuk, Curr. Opin. Colloid Interface Sci. 15 (2010) 217

L. M. C. Sagis, E. Scholten, Trends Food Sci. Technol. 37 (2014) 59

R. Miller, J. K. Ferri, A. Javadi, J. Krägel, N. Mucic, R. Wüstneck, Colloid. Polym. Sci. 288 (2010) 937

A. M. Ghribi, I. M. Gafsi A. Sila, C. Blecker, S. Danthine, H. Attia, A. Bougatef, S. Besbes, Food Chem. 187 (2015) 322

R. S. H. Lam, M. T. Nickerson, Food Chem. 141 (2013) 975

B. Ozturk, D. J. McClements, Curr. Opin. Food Sci. 7 (2016) 1

B. S. Murray, Curr. Opin. Colloid Interface Sci. 16 (2011) 27

V. P. Ruíz-Henestrosa, C. C. Sánchez, J. J. Pedroche, F. Millán, J. M. R. Patino, Food Hydrocolloids 23 (2009) 377

V. P. Ruíz-Henestrosa, C. C. Sánchez, M. M. Yust, J. J. Pedroche, F. Millán, J. M. R. Patino, J. Agric. Food Chem. 55 (2007) 1536

J. M. Conde, J. M. R. Patino, Food Hydrocolloids 21 (2007) 212

F. Tamm, S. Drusch, Food Hydrocolloids 63 (2017) 8

O. L. Tavano, J. Mol. Catal. B: Enzym. 90 (2013) 1

X. D. Sun, Int. J. Food Sci. Technol. 46 (2011) 2447

N. A. Avramenko, N. H. Low, M. T. Nickerson, Food Res. Int. 51 (2013) 162

Ž. Vaštag, Lj. Popović, S. Popović, V. Krimer, D. Peričin, Food Chem. 124 (2011) 1316

Lj. Popović, D. Peričin, Ž. Vaštag, S. Popović, V. Krimer, A. Torbica, J. Am. Oil Chem. Soc. 90 (2013) 1157

Ž. Vaštag, Lj. Popović, S. Popović, D. Peričin, V. Krimer, Food Bioprod. Process. 88 (2010) 277

S. Bučko, J. Katona, Lj. Popović, L. Petrović, J. Milinković, Food Hydrocolloids 60 (2016) 271

K. Tsumura, W. Kugimiya, N. Bando, M. Hiemori, T. Ogawa, Food Sci. Technol. Res. 5 (1999) 171

O. H. Lowry, N. J. Rosenbrough, A. L. Fair, R. J. Randall, J. Biol. Chem. 193 (1951) 265

J. M. R. Patino, J. M. Conde, H. M. Linares, J. J. P. Jímenez, C. C. Sánchez, V. Pizones, F. M. Rodríguez, Food Hydrocolloids 21 (2007) 782

J. Maldonado-Valderrama, V. B. Fainerman, E. Aksenenko, M. J. Gálvez-Ruiza, M. A. Cabrerizo-Vílcheza, R. Miller, Colloids Surfaces, A 261 (2005) 85

B. Yuan, J. Ren, M. Zhao, D. Luo, L. Gu, LWT Food Sci. Technol. 46 (2012) 453

K. D. Martínez, C. C. Sánchez, J. M. R. Patino, A. M. R. Pilosof, Food Hydrocolloids 23 (2009) 2149

J. P. Davis, D. Doucet, E. A. Foegeding, J. Colloid Interface Sci. 288 (2005) 412.




DOI: https://doi.org/10.2298/JSC171120042B

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