A novel compound isolated from Sclerochloa dura has anti-inflammatory effects

Syed Majid Bukhari, Astrid Jullumstrø Feuerherm, Hanna Maja Tunset, Stian Moe Isaksen, Mari Sæther, Thor Håkon Thvedt, Susana Villa Gonzalez, Rudolf Schmid, Anders Brunsvik, Erik Fuglset, Bojan Zlatković, Berit Johansen, Nebojša Simić

Abstract


The activation of PLA2 by means of pro-inflammatory cytokines results in the subsequent release of arachidonic acid (AA) and generates eicosanoids, which further propagate inflammation. By 6the cyclooxygenases (COX1/2) responsible for the enzymatic conversion of AA to eicosanoids, the non-steroidal anti-inflammatory drugs are effective in relieving the pain and discomfort of inflammation. By using AA release assay as a guide for biological and anti-inflammatory activity, novel compound 1-O-(3-O-linolenoyl-6-deoxy-6-sulfo-α-D-glucopyranosyl)-glycerol (1) together with five known compounds isovitexin, byzantionoside B, tricin 4-O-(erythro-β-guaiacylglyceryl) ether 7-O-β-glucopyranoside, 1-O-feruloyl glycerol and tricin 7-glucoside were isolated from the methanol extract of the aerial parts of Sclerochloa dura using LC techniques (Sephadex LH-20 column chromatography, preparative HPLC and semi-preparative HPLC). All isolated compounds were identified using spectroscopic NMR spectroscopy and MS spectrometry. Novel compound (1) was found to be an effective inhibitor of AA release with an IC50 value of 0.09 ± 0.03 mg mL-1.

Keywords


arachidonic acid; anti-inflammatory activity; PLA2 inhibitor; Sephadex LH-20; HPLC; Cell line SW982.



DOI: https://doi.org/10.2298/JSC150119055B

Copyright (c) 2015 Journal of the Serbian Chemical Society - J. Serb. Chem. Soc.

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