Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts Scientific paper

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Wikimedia - Original book source: Prof. Dr. Otto Wilhelm Thomé Flora von Deutschland, Österreich und der Schweiz 1885, Gera, Germany Permission granted to use under GFDL by Kurt Stueber Source: www.biolib.de
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Published: Jun 29, 2021
DOI: https://doi.org/10.2298/JSC201111013G
Keywords:
antioxidant activity extraction escins coumarin glycosides phenolic compounds

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Tanja Gagić
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia
https://orcid.org/0000-0003-0331-3531
Željko Knez
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia and Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia
Mojca Škerget
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia
https://orcid.org/0000-0001-5619-3487

Abstract

Subcritical water extraction of horse chestnut (Aesculus hippocas­tanum) parts, such as seeds, seed shell, bark and leaves has been performed in a batch reactor at temperatures of 150, 200 and 250 °C and extraction times of 5, 15 and 30 min. The obtained extracts were analyzed by spectrophotometric methods to determine the total phenols, total carbohydrates and antioxidant act­ivity. Furthermore, the compounds detected in the extracts, such as triterpene saponins (escins), coumarin glycosides (esculin and fraxin), phenolic com­pounds (chlorogenic, neochlorogenic and gallic acids) and furfurals (5-hydro­xy­methyfurfural, furfural, and 5-methylfufrual) were quantified using HPLC. High amount of escins was obtained in the seed extracts, while the highest amounts of esculin and fraxin were obtained in bark extracts. The total phenol content was the highest in shell and bark extracts, which implies that these extracts gave the highest antioxidant activity.

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How to Cite
[1]
T. Gagić, Željko Knez, and M. Škerget, “Subcritical water extraction of horse chestnut (Aesculus hippocastanum) tree parts: Scientific paper”, J. Serb. Chem. Soc., vol. 86, no. 6, pp. 603–613, Jun. 2021.
Issue
Vol. 86 No. 6 (2021)
Section
Chemical Engineering
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