Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase

Authors

  • Xin Wang School of Science, Shenyang University of Technology, Shenyang 110870
  • Shi Yu Sun School of Science, Shenyang University of Technology, Shenyang 110870
  • Zi Jun Ni School of Science, Shenyang University of Technology, Shenyang 110870
  • Zhao Xing Li School of Science, Shenyang University of Technology, Shenyang 110870
  • Jia Bao School of Science, Shenyang University of Technology, Shenyang 110870

DOI:

https://doi.org/10.2298/JSC171004022W

Keywords:

immobilized laccase, polycyclic aromatic hydrocarbons (PAHs), soil remediation, nylon net, chitosan

Abstract

The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 volume ratio of soil to PAH solution. Sub­se­quently, the adsorption–cross-link composite immobilization method was applied to immobilize laccase derived from fungi onto nylon net and chitosan, respectively. These two kinds of immobilized laccase were used in the degrad­ation of pyrene (Pyr) and benzo[a]pyrene (BaP), and their degradation effi­ci­encies under dif­fer­ent temperature and pH conditions were investigated. Con­se­quently, the optimal laboratory experimental parameters were determined as follows: first, compared with free laccase, the degradation rates of Pyr and BaP by immo­bilized laccase increased by around 10–30 %; second, the degradation effi­ciency of chitosan as a carrier of immobilized laccase was much better than that of nylon net as a carrier of the immobilized laccase; finally, when the tem­perature was set at 40 °C and the pH was set at 4, the degradation efficiency achieved by immobilized laccase was the best.

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Published

2018-06-05

How to Cite

[1]
X. Wang, S. Y. Sun, Z. J. Ni, Z. X. Li, and J. Bao, “Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase”, J. Serb. Chem. Soc., vol. 83, no. 5, pp. 549-559, Jun. 2018.

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Section

Biochemistry & Biotechnology