Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion

Jelena Živković, Katarina Savikin, Nemanja Stanisavljević, Gordana Zdunić, Tatjana Stanojković, Jelena Samardžić

Abstract


Decoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of food matrix in order to determine changes in polyphenol content and antiproliferative activity. Total phenolic and total proanthocyanidins content were determined using spectrophotometrical methods, and phenolic compounds were analyzed by RP-HPLC/DAD before and after digestion. Chlorogenic acid and phlorizin dihydrate were the major identified compounds. Addition of food matrix immediately decreased content of individual and total phenolics. After digestion they were slightly elevated but still lower than in initial samples. Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa), human colon carcinoma (LS174) and human fetal lung fibroblast (MRC-5) cells. Exhibited growth inhibition was not correlated with the content of phenolics in tested samples indicating that it could not be explained solely by their content. With the exception of apple decoction effect on HeLa cells, digestion process resulted in significant increase of antiproliferative activity.


Keywords


bioaccessibility; phenolics; food matrix; fruit

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DOI: https://doi.org/10.2298/JSC180604073Z

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