HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua Scientific paper

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Jovana Stanković Jeremić
https://orcid.org/0000-0003-1560-5822
Dejan Gođevac
https://orcid.org/0000-0002-0555-581X
Stefan Ivanović
https://orcid.org/0000-0002-3291-9818
Katarina Simić
https://orcid.org/0000-0001-5060-8936
Antoaneta Trendafilova
https://orcid.org/0000-0001-5273-176X
Milica Aćimović
https://orcid.org/0000-0002-5346-1412
Slobodan Milosavljević
https://orcid.org/0000-0002-0925-327X

Abstract

The application of high performance thin layer chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is des­cribed. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate ana­lysis. Principal component analysis and orthogonal partial least squares dis­criminant analysis confirmed the differentiation of samples belonging to veget­ative phase, flowering stage, and seed formation stage of the plant develop­ment. The obtained results showed that the HPTLC-based metabolomics appro­ach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to gas chromatography–mass spec­trometry and nuclear magnetic resonance-based metabolomics.

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How to Cite
[1]
J. Stanković Jeremić, “HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua: Scientific paper”, J. Serb. Chem. Soc., vol. 87, no. 11, pp. 1237–1244, Oct. 2022.
Section
Organic Chemistry

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