HPTLC-based metabolomics for the investigation of metabolic changes during plant development: the case study of Artemisia annua Scientific paper

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Jovana Stanković Jeremić
Dejan Gođevac
Stefan Ivanović
Katarina Simić
Antoaneta Trendafilova
Milica Aćimović
Slobodan Milosavljević


The application of High Performance Thin Layer Chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is described. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate analysis. Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of samples belonging to vegetative phase, flowering stage, and seed formation stage of the plant development. The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to GC-MS and NMR-based metabolomics.


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How to Cite
J. Stanković Jeremić, “HPTLC-based metabolomics for the investigation of metabolic changes during plant development: the case study of Artemisia annua: Scientific paper”, J. Serb. Chem. Soc., Mar. 2022.
Organic Chemistry


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