HPTLC-based metabolomics for the investigation of metabolic changes during plant development: the case study of Artemisia annua Scientific paper

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Jovana Stanković Jeremić
https://orcid.org/0000-0003-1560-5822
Dejan Gođevac
https://orcid.org/0000-0002-0555-581X
Stefan Ivanović
https://orcid.org/0000-0002-3291-9818
Katarina Simić
https://orcid.org/0000-0001-5060-8936
Antoaneta Trendafilova
https://orcid.org/0000-0001-5273-176X
Milica Aćimović
https://orcid.org/0000-0002-5346-1412
Slobodan Milosavljević
https://orcid.org/0000-0002-0925-327X

Abstract

The application of High Performance Thin Layer Chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is described. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate analysis. Principal component analysis and Orthogonal Partial Least Squares Discriminant Analysis confirmed the discrimination of samples belonging to vegetative phase, flowering stage, and seed formation stage of the plant development. The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to GC-MS and NMR-based metabolomics.

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How to Cite
[1]
J. Stanković Jeremić, “HPTLC-based metabolomics for the investigation of metabolic changes during plant development: the case study of Artemisia annua: Scientific paper”, J. Serb. Chem. Soc., Mar. 2022.
Section
Organic Chemistry

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