HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of  Artemisia annua: Scientific paper

Jovana Stanković Jeremić; Dejan  Gođevac; Stefan Ivanović; Katarina Simić; Antoaneta Trendafilova; Milica Aćimović; Slobodan Milosavljević

doi:10.2298/JSC210507007S

PDF (1.821 kB) Supplementary Material (3.83 MB)

Published: Oct 10, 2022

Updated: 10.10.2022

DOI: https://doi.org/10.2298/JSC210507007S

Keywords:

plant metabolomics PCA OPLS-DA

Jovana Stanković Jeremić

University of Belgrade Institute of Chemistry, Technology and Metallurgy National Institute of the Republic of Serbia, Belgrade, Serbia

https://orcid.org/0000-0003-1560-5822

Dejan Gođevac

University of Belgrade – Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, 11000 Belgrade, Serbia

https://orcid.org/0000-0002-0555-581X

Stefan Ivanović

University of Belgrade – Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, 11000 Belgrade, Serbia

https://orcid.org/0000-0002-3291-9818

Katarina Simić

University of Belgrade – Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, 11000 Belgrade, Serbia

https://orcid.org/0000-0001-5060-8936

Antoaneta Trendafilova

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

https://orcid.org/0000-0001-5273-176X

Milica Aćimović

Institute of Field and Vegetable crops, 21000 Novi Sad, Serbia

https://orcid.org/0000-0002-5346-1412

Slobodan Milosavljević

University of Belgrade - Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia

https://orcid.org/0000-0002-0925-327X

Abstract

The application of high performance thin layer chromatography (HPTLC)-based non-targeted metabolomics as a holistic approach to compare fingerprints of metabolite changes during Artemisia annua development is described. Images of HPTLC chromatograms obtained after derivatization with anisaldehyde-sulphuric acid reagent were used as a dataset for multivariate analysis. Principal component analysis and orthogonal partial least squares discriminant analysis confirmed the differentiation of samples belonging to vegetative phase, flowering stage, and seed formation stage of the plant development. The obtained results showed that the HPTLC-based metabolomics approach can be a very reliable technique for the investigation of metabolic changes during plant development, complementary to gas chromatography–mass spectrometry and nuclear magnetic resonance-based metabolomics.

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[1]

J. Stanković Jeremić, “HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua: Scientific paper”, J. Serb. Chem. Soc., vol. 87, no. 11, pp. 1237–1244, Oct. 2022.

Issue

Vol. 87 No. 11 (2022)

Section

Organic Chemistry

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution license 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

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HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua Scientific paper